Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents It is provided in pKa is an acid dissociation constant used to describe the acidity of a particular molecule Its value is directly related to the structure of the given compound The constant changes depending on the solvent the compound is used in Typically, organic chemists compare the various values from their determination in water, DMSO and the gas phase and use these to predict aIssue in Honor of Prof António M d'A Rocha Gonçalves ARKIVOC 10 (v) 91 ISSN Page 84 ©ARKAT USA, Inc Results and Discussion 3 The synthetic versatility of the easily accessible amidines 3 and the biological importance of the guanidine substituent, prompted us to study the reaction of imidate 1 with guanidine (Scheme 2), aiming at the synthesis of amidine 3
2
